Detection of Kyuri Green Mottle Mosaic Virus from Soil by the Immunocapture Reverse Transcription Loop-mediated Isothermal Amplification Reaction

Differential detection of Wheat yellow mosaic virus, Japanese soil-borne wheat mosaic virus and Chinese wheat mosaic virus by reverse transcription loop-mediated isothermal amplification reaction.

A differential detection method for three wheat viruses: Wheat yellow mosaic virus (WYMV), Japanese soil-borne mosaic virus (JSBWMV) and Chinese wheat mosaic virus (CWMV) using reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction was developed. All three primer sets, which were designed from the genome sequences of WYMV, JSBWMV and CWMV respectively, worked most effic...

Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus by Reverse Transcription Loop-Mediated Isothermal Amplification

The fragment of the membrane protein M gene with high conservation and specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was chosen to be the target region, according to which six special primers were designed successfully. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65° for only 45 min with the...

Rapid detection of Lassa virus by reverse transcription-loop-mediated isothermal amplification.

In this study, a simple one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Lassa virus (LASV) was established. The two primer sets were designed to detect LASV circulating in Sierra Leone and northeastern Nigeria. The RT-LAMP assay using these primer sets was able to detect 100 copies of the in vitro transcribed artificial LASV RNA withi...

Detection of Raspberry ringspot virus Using Reverse Transcription Loop-mediated Isothermal Amplification

Raspberry ringspot virus (RpRSV), which has not occurred in Japan, infects many plants such as raspberry, gooseberry, cherry, grapevine, and strawberry. Biological assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) have been introduced for the detection of RpRSV in postentry quarantine in Japan. We studied reverse transcription loop-me...

Rapid Detection of West Nile Virus for Loop-Mediated Isothermal Amplification Real-Time Reverse Transcription